Detailed information of TA system TA119329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 41645..42302
• Replicon: plasmid pIncAC2
• Accession: NZ_CP036192
• Organism: Klebsiella pneumoniae strain BA34918

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: EX353_RS28560
• Protein ID: WP_000270043.1
• Coordinates: 41952..42302 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EX353_RS28555
• Protein ID: WP_000124640.1
• Coordinates: 41645..41947 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX353_RS28505	37279..37707	+	429	WP_000591076.1	hypothetical protein	-
EX353_RS28510	37765..38124	+	360	WP_000422769.1	hypothetical protein	-
EX353_RS28515	38124..38570	+	447	WP_000919343.1	hypothetical protein	-
EX353_RS28520	38567..39085	+	519	WP_000210757.1	nitrite reductase	-
EX353_RS28525	39085..39315	+	231	WP_000972665.1	hypothetical protein	-
EX353_RS28530	39302..40159	+	858	WP_001167036.1	hypothetical protein	-
EX353_RS32900	40071..40376	+	306	WP_171820680.1	hypothetical protein	-
EX353_RS28540	40379..40906	+	528	WP_004201083.1	thermonuclease family protein	-
EX353_RS28545	40964..41236	+	273	WP_001043046.1	HU family DNA-binding protein	-
EX353_RS28550	41325..41618	+	294	WP_001239998.1	hypothetical protein	-
EX353_RS28555	41645..41947	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
EX353_RS28560	41952..42302	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EX353_RS28565	42465..43013	+	549	WP_001061573.1	hypothetical protein	-
EX353_RS28575	43354..43548	+	195	WP_000343597.1	hypothetical protein	-
EX353_RS28580	43559..43930	+	372	WP_000516918.1	hypothetical protein	-
EX353_RS28585	43923..44393	+	471	WP_001281821.1	hypothetical protein	-
EX353_RS28590	44408..44743	-	336	WP_000683477.1	hypothetical protein	-
EX353_RS28595	44840..45328	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
EX353_RS28600	45331..45828	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..165052	165052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T119329 WP_000270043.1 NZ_CP036192:c42302-41952 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T119329 NZ_CP036192:c42302-41952 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT119329 WP_000124640.1 NZ_CP036192:c41947-41645 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT119329 NZ_CP036192:c41947-41645 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9