Detailed information of TA system TA119285

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108497..108750
• Replicon: plasmid p1_025970
• Accession: NZ_CP036180
• Organism: Escherichia coli strain WCHEC025970

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EYD11_RS25635
• Protein ID: WP_001312851.1
• Coordinates: 108601..108750 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	108497..108556 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYD11_RS25605	105224..105970	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
EYD11_RS25610	106025..106585	+	561	WP_000139328.1	fertility inhibition protein FinO	-
EYD11_RS25615	106717..106917	+	201	WP_015059022.1	hypothetical protein	-
EYD11_RS25620	107303..107902	+	600	WP_032083981.1	hypothetical protein	-
EYD11_RS25625	108066..108296	+	231	WP_001736714.1	hypothetical protein	-
-	108497..108556	-	60	NuclAT_1	-	Antitoxin
-	108497..108556	-	60	NuclAT_1	-	Antitoxin
-	108497..108556	-	60	NuclAT_1	-	Antitoxin
-	108497..108556	-	60	NuclAT_1	-	Antitoxin
EYD11_RS25635	108601..108750	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
EYD11_RS25640	109034..109282	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / blaTEM-1B / rmtB	-	1..109593	109593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T119285 WP_001312851.1 NZ_CP036180:108601-108750 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T119285 NZ_CP036180:108601-108750 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT119285 NZ_CP036180:c108556-108497 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin