Detailed information of TA system TA119281

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69009..69279
• Replicon: plasmid p1_025970
• Accession: NZ_CP036180
• Organism: Escherichia coli strain WCHEC025970

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EYD11_RS25390
• Protein ID: WP_001312861.1
• Coordinates: 69121..69279 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69009..69072 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYD11_RS25365	64720..65247	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
EYD11_RS25370	65305..65538	+	234	WP_000006003.1	DUF905 family protein	-
EYD11_RS25375	65599..67622	+	2024	Protein_81	ParB/RepB/Spo0J family partition protein	-
EYD11_RS25380	67691..68125	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EYD11_RS25385	68122..68841	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	68853..69077	+	225	NuclAT_0	-	-
-	68853..69077	+	225	NuclAT_0	-	-
-	68853..69077	+	225	NuclAT_0	-	-
-	68853..69077	+	225	NuclAT_0	-	-
EYD11_RS26680	68862..69041	-	180	WP_001309233.1	hypothetical protein	-
-	69009..69072	-	64	-	-	Antitoxin
EYD11_RS25390	69121..69279	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EYD11_RS26835	69517..69894	-	378	Protein_86	hypothetical protein	-
EYD11_RS25410	70194..70490	+	297	WP_001272251.1	hypothetical protein	-
EYD11_RS25415	70601..71422	+	822	WP_001234445.1	DUF945 domain-containing protein	-
EYD11_RS25420	71719..72366	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
EYD11_RS25425	72643..73026	+	384	WP_000124981.1	relaxosome protein TraM	-
EYD11_RS25430	73217..73903	+	687	WP_015059009.1	PAS domain-containing protein	-
EYD11_RS25435	73997..74224	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / blaTEM-1B / rmtB	-	1..109593	109593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T119281 WP_001312861.1 NZ_CP036180:69121-69279 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T119281 NZ_CP036180:69121-69279 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT119281 NZ_CP036180:c69072-69009 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin