Detailed information of TA system TA119273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3712733..3713427
• Replicon: chromosome
• Accession: NZ_CP036177
• Organism: Escherichia coli strain WCHEC025970

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: EYD11_RS18110
• Protein ID: WP_001263489.1
• Coordinates: 3712733..3713131 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: EYD11_RS18115
• Protein ID: WP_000554758.1
• Coordinates: 3713134..3713427 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3708321..3708401	-	81	NuclAT_11	-	-
-	3708321..3708401	-	81	NuclAT_11	-	-
-	3708321..3708401	-	81	NuclAT_11	-	-
-	3708321..3708401	-	81	NuclAT_11	-	-
EYD11_RS18085	3708997..3709455	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
EYD11_RS18090	3709716..3711173	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
EYD11_RS18095	3711230..3711751	-	522	Protein_3515	peptide chain release factor H	-
EYD11_RS18100	3711747..3711953	-	207	Protein_3516	RtcB family protein	-
EYD11_RS18105	3712271..3712723	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
EYD11_RS18110	3712733..3713131	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EYD11_RS18115	3713134..3713427	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EYD11_RS18120	3713479..3714534	-	1056	WP_001226164.1	DNA polymerase IV	-
EYD11_RS18125	3714605..3715390	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
EYD11_RS18130	3715362..3717074	+	1713	Protein_3522	flagellar biosynthesis protein FlhA	-
EYD11_RS18135	3717298..3717795	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T119273 WP_001263489.1 NZ_CP036177:c3713131-3712733 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T119273 NZ_CP036177:c3713131-3712733 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT119273 WP_000554758.1 NZ_CP036177:c3713427-3713134 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT119273 NZ_CP036177:c3713427-3713134 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3