Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1843109..1843611 Replicon chromosome
Accession NZ_CP036177
Organism Escherichia coli strain WCHEC025970

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EYD11_RS08760 Protein ID WP_000767829.1
Coordinates 1843357..1843611 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EYD11_RS08755 Protein ID WP_001259255.1
Coordinates 1843109..1843360 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYD11_RS08730 1838286..1839353 - 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EYD11_RS08735 1839353..1840423 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
EYD11_RS08740 1840420..1841724 - 1305 WP_001446927.1 histidinol dehydrogenase -
EYD11_RS08745 1841730..1842629 - 900 WP_032329340.1 ATP phosphoribosyltransferase -
EYD11_RS08750 1842775..1842825 - 51 WP_001364200.1 his operon leader peptide -
EYD11_RS08755 1843109..1843360 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EYD11_RS08760 1843357..1843611 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EYD11_RS08765 1843694..1844518 + 825 WP_000754749.1 SDR family oxidoreductase -
EYD11_RS08770 1844564..1845493 + 930 WP_000803351.1 LysR family transcriptional regulator -
EYD11_RS08775 1845708..1845770 + 63 WP_010723108.1 membrane protein YoeI -
EYD11_RS08780 1845760..1847118 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EYD11_RS08785 1847297..1848355 + 1059 WP_000492321.1 transport protein YeeE -
EYD11_RS08790 1848369..1848596 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T119260 WP_000767829.1 NZ_CP036177:1843357-1843611 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T119260 NZ_CP036177:1843357-1843611 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT119260 WP_001259255.1 NZ_CP036177:1843109-1843360 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT119260 NZ_CP036177:1843109-1843360 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References