Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3093395..3093978 Replicon chromosome
Accession NZ_CP036164
Organism Janibacter limosus strain P3-3-X1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EXU32_RS14835 Protein ID WP_130630601.1
Coordinates 3093395..3093673 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag EXU32_RS14840 Protein ID WP_130630602.1
Coordinates 3093694..3093978 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXU32_RS14805 3088909..3089421 + 513 WP_130630595.1 GyrI-like domain-containing protein -
EXU32_RS14810 3089449..3090294 - 846 WP_130630596.1 SDR family oxidoreductase -
EXU32_RS14815 3090291..3090605 - 315 WP_130630597.1 hypothetical protein -
EXU32_RS14820 3090681..3091859 + 1179 WP_130630598.1 thiolase family protein -
EXU32_RS14825 3091859..3092605 + 747 WP_130630599.1 enoyl-CoA hydratase/isomerase family protein -
EXU32_RS14830 3092786..3093277 - 492 WP_130630600.1 DUF4395 domain-containing protein -
EXU32_RS14835 3093395..3093673 + 279 WP_130630601.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EXU32_RS14840 3093694..3093978 + 285 WP_130630602.1 HigA family addiction module antidote protein Antitoxin
EXU32_RS14845 3094036..3094503 + 468 WP_130630603.1 YbaK/EbsC family protein -
EXU32_RS14850 3094706..3095464 - 759 WP_055989346.1 SDR family NAD(P)-dependent oxidoreductase -
EXU32_RS14855 3095596..3096552 + 957 WP_130630604.1 5,10-methylenetetrahydrofolate reductase -
EXU32_RS14860 3096593..3097432 + 840 WP_130630605.1 SMP-30/gluconolactonase/LRE family protein -
EXU32_RS14865 3097550..3098707 + 1158 WP_130630606.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(2-91)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10664.19 Da        Isoelectric Point: 9.5922

>T119234 WP_130630601.1 NZ_CP036164:3093395-3093673 [Janibacter limosus]
VIVSFRHKGLERLYRTETKKGVQAAHVPKLLRILSALDVAQGPADLEIPSFRTHRLKGDLTGHHSIWVNGNWRITFRFIE
SDVELVDYQDYH

Download         Length: 279 bp

>T119234 NZ_CP036164:3093395-3093673 [Janibacter limosus]
GTGATCGTCAGTTTCCGACACAAGGGACTGGAGCGGTTGTACCGCACCGAGACGAAGAAGGGTGTCCAGGCGGCGCACGT
CCCCAAGCTGCTGCGCATCCTGTCGGCCCTGGACGTCGCCCAAGGACCGGCCGACCTTGAGATCCCGTCCTTTCGCACTC
ATCGACTCAAGGGTGACCTGACGGGCCACCACTCCATATGGGTCAACGGCAACTGGCGCATCACCTTCCGATTCATCGAG
TCGGATGTCGAGCTTGTCGATTATCAGGACTATCACTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 9906.43 Da        Isoelectric Point: 10.6934

>AT119234 WP_130630602.1 NZ_CP036164:3093694-3093978 [Janibacter limosus]
MMKNPVHPGEILREDVLADLGLTVSEAANRLGVSRVSLSRVLHGHARISSTLAIRLEAAGVGTASSWLAMQSAHDLSVER
AAGIPKVQRLTSVA

Download         Length: 285 bp

>AT119234 NZ_CP036164:3093694-3093978 [Janibacter limosus]
ATGATGAAGAATCCGGTCCACCCCGGCGAGATCCTGCGCGAGGACGTTTTGGCTGACCTCGGCCTCACCGTCAGCGAGGC
CGCCAATCGCCTCGGCGTCTCGCGGGTGTCGTTGAGCCGGGTCCTGCACGGCCACGCCAGGATCAGCTCCACCTTGGCGA
TCCGGCTCGAGGCTGCCGGCGTCGGCACCGCGAGCTCGTGGCTGGCCATGCAGTCTGCCCACGACCTGTCCGTCGAACGT
GCGGCGGGCATCCCCAAGGTGCAGCGCCTCACGTCGGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References