Detailed information of TA system TA119100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1109192..1109919
• Replicon: chromosome
• Accession: NZ_CP035944
• Organism: Escherichia coli strain AR24.2b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: EXT04_RS05440
• Protein ID: WP_000547564.1
• Coordinates: 1109192..1109503 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXT04_RS05445
• Protein ID: WP_000126294.1
• Coordinates: 1109500..1109919 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXT04_RS05410	1104334..1106043	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
EXT04_RS05415	1106053..1106595	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
EXT04_RS05420	1106595..1107362	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EXT04_RS05425	1107359..1107769	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
EXT04_RS05430	1107762..1108232	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EXT04_RS05435	1108257..1109018	+	762	WP_001026446.1	hypothetical protein	-
EXT04_RS05440	1109192..1109503	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
EXT04_RS05445	1109500..1109919	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EXT04_RS05450	1110033..1111457	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
EXT04_RS05455	1111466..1112923	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EXT04_RS05460	1113183..1114193	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
EXT04_RS05465	1114342..1114869	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T119100 WP_000547564.1 NZ_CP035944:1109192-1109503 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T119100 NZ_CP035944:1109192-1109503 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT119100 WP_000126294.1 NZ_CP035944:1109500-1109919 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT119100 NZ_CP035944:1109500-1109919 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5