Detailed information of TA system TA119094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 7292..7877
• Replicon: plasmid p2_060092
• Accession: NZ_CP035937
• Organism: Acinetobacter cumulans strain WCHAc060092

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C9E88_RS17280
• Protein ID: WP_000897307.1
• Coordinates: 7557..7877 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C9E88_RS17275
• Protein ID: WP_000369781.1
• Coordinates: 7292..7564 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E88_RS17245	2374..2730	+	357	WP_004967307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C9E88_RS17250	2723..3025	+	303	WP_001140620.1	XRE family transcriptional regulator	-
C9E88_RS17255	3107..3382	-	276	WP_004967304.1	hypothetical protein	-
C9E88_RS17260	3583..4194	+	612	WP_015060246.1	recombinase family protein	-
C9E88_RS17265	4384..5268	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C9E88_RS17270	5324..6799	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C9E88_RS17275	7292..7564	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C9E88_RS17280	7557..7877	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E88_RS17285	8036..8677	-	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
C9E88_RS17290	8743..9930	+	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
C9E88_RS17650	10154..10297	-	144	WP_167514224.1	hypothetical protein	-
C9E88_RS17295	10310..10864	-	555	WP_068975321.1	helix-turn-helix domain-containing protein	-
C9E88_RS17300	10868..11812	-	945	WP_068912167.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / mph(E) / msr(E) / tet(39) / sul2	-	1..18761	18761
	-	inside	IS/Tn	aph(3'')-Ib / aph(6)-Id / mph(E) / msr(E) / tet(39)	-	28..9930	9902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T119094 WP_000897307.1 NZ_CP035937:c7877-7557 [Acinetobacter cumulans]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T119094 NZ_CP035937:c7877-7557 [Acinetobacter cumulans]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT119094 WP_000369781.1 NZ_CP035937:c7564-7292 [Acinetobacter cumulans]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT119094 NZ_CP035937:c7564-7292 [Acinetobacter cumulans]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3