Detailed information of TA system TA119093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2374..3025
• Replicon: plasmid p2_060092
• Accession: NZ_CP035937
• Organism: Acinetobacter cumulans strain WCHAc060092

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BC17
• Locus tag: C9E88_RS17245
• Protein ID: WP_004967307.1
• Coordinates: 2374..2730 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C9E88_RS17250
• Protein ID: WP_001140620.1
• Coordinates: 2723..3025 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E88_RS17230	514..1317	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
C9E88_RS17235	1317..2153	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
C9E88_RS17245	2374..2730	+	357	WP_004967307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E88_RS17250	2723..3025	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
C9E88_RS17255	3107..3382	-	276	WP_004967304.1	hypothetical protein	-
C9E88_RS17260	3583..4194	+	612	WP_015060246.1	recombinase family protein	-
C9E88_RS17265	4384..5268	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C9E88_RS17270	5324..6799	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C9E88_RS17275	7292..7564	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C9E88_RS17280	7557..7877	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / mph(E) / msr(E) / tet(39) / sul2	-	1..18761	18761
	-	inside	IS/Tn	aph(3'')-Ib / aph(6)-Id / mph(E) / msr(E) / tet(39)	-	28..9930	9902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13601.62 Da
• Isoelectric Point: 6.4648

>T119093 WP_004967307.1 NZ_CP035937:2374-2730 [Acinetobacter cumulans]
MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGSKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T119093 NZ_CP035937:2374-2730 [Acinetobacter cumulans]
ATGTGGATAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTAGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCTTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCAGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT119093 WP_001140620.1 NZ_CP035937:2723-3025 [Acinetobacter cumulans]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT119093 NZ_CP035937:2723-3025 [Acinetobacter cumulans]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATCTCTCAAAAGGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGCGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7T4TW67