Detailed information of TA system TA119044

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4780468..4780839
• Replicon: chromosome
• Accession: NZ_CP035884
• Organism: Escherichia coli strain LSN03-016011/A

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: EW995_RS23030
• Protein ID: WP_001443846.1
• Coordinates: 4780690..4780839 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4780468..4780646 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW995_RS23005 (4776423)	4776423..4777796	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
EW995_RS23010 (4777925)	4777925..4778860	-	936	WP_001157405.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EW995_RS23015 (4778912)	4778912..4780147	-	1236	WP_000040852.1	site-specific integrase	-
EW995_RS23020 (4780149)	4780149..4780364	-	216	WP_000079604.1	excisionase XisR	-
- (4780468)	4780468..4780646	+	179	NuclAT_0	-	Antitoxin
- (4780468)	4780468..4780646	+	179	NuclAT_0	-	Antitoxin
- (4780468)	4780468..4780646	+	179	NuclAT_0	-	Antitoxin
- (4780468)	4780468..4780646	+	179	NuclAT_0	-	Antitoxin
EW995_RS23025 (4780443)	4780443..4780652	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
EW995_RS23030 (4780690)	4780690..4780839	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EW995_RS23035 (4780896)	4780896..4781705	-	810	WP_134737085.1	recombination protein RecT	-
EW995_RS23040 (4781698)	4781698..4784298	-	2601	WP_001702664.1	exodeoxyribonuclease VIII	-
EW995_RS23045 (4784400)	4784400..4784675	-	276	WP_001314664.1	hypothetical protein	-
EW995_RS23050 (4784750)	4784750..4784920	-	171	WP_134737086.1	YdaE family protein	-
EW995_RS23055 (4784920)	4784920..4785141	-	222	WP_000560230.1	killing protein KilR	-
EW995_RS23060 (4785550)	4785550..4785702	-	153	WP_001169150.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4778912..4827768	48856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T119044 WP_001443846.1 NZ_CP035884:c4780839-4780690 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T119044 NZ_CP035884:c4780839-4780690 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT119044 NZ_CP035884:4780468-4780646 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin