Detailed information of TA system TA119030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1305013..1305740
• Replicon: chromosome
• Accession: NZ_CP035884
• Organism: Escherichia coli strain LSN03-016011/A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: EW995_RS06305
• Protein ID: WP_000547564.1
• Coordinates: 1305429..1305740 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EW995_RS06300
• Protein ID: WP_000126294.1
• Coordinates: 1305013..1305432 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW995_RS06280 (1300063)	1300063..1300590	-	528	WP_001078777.1	electron transport protein HydN	-
EW995_RS06285 (1300739)	1300739..1301749	-	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
EW995_RS06290 (1302009)	1302009..1303466	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EW995_RS06295 (1303475)	1303475..1304899	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
EW995_RS06300 (1305013)	1305013..1305432	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EW995_RS06305 (1305429)	1305429..1305740	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
EW995_RS06310 (1305914)	1305914..1306675	-	762	WP_001026446.1	hypothetical protein	-
EW995_RS06315 (1306700)	1306700..1307170	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EW995_RS06320 (1307163)	1307163..1307573	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
EW995_RS06325 (1307570)	1307570..1308337	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EW995_RS06330 (1308337)	1308337..1308879	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
EW995_RS06335 (1308889)	1308889..1310598	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T119030 WP_000547564.1 NZ_CP035884:c1305740-1305429 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T119030 NZ_CP035884:c1305740-1305429 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT119030 WP_000126294.1 NZ_CP035884:c1305432-1305013 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT119030 NZ_CP035884:c1305432-1305013 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5