Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 92387..92799 | Replicon | plasmid pWS0115A_186 |
| Accession | NZ_CP035883 | ||
| Organism | Escherichia coli strain WS0115A | ||
Toxin (Protein)
| Gene name | hok | Uniprot ID | - |
| Locus tag | EW996_RS23950 | Protein ID | WP_223200913.1 |
| Coordinates | 92387..92509 (-) | Length | 41 a.a. |
Antitoxin (RNA)
| Gene name | pndB | ||
| Locus tag | - | ||
| Coordinates | 92594..92799 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EW996_RS23925 (88485) | 88485..89294 | - | 810 | Protein_112 | IS256 family transposase | - |
| EW996_RS23930 (89377) | 89377..89781 | + | 405 | WP_000839179.1 | transposase | - |
| EW996_RS23935 (89778) | 89778..90125 | + | 348 | WP_000612626.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EW996_RS23940 (90174) | 90174..91712 | + | 1539 | WP_000099202.1 | IS66-like element ISEc22 family transposase | - |
| EW996_RS23945 (91748) | 91748..92209 | - | 462 | Protein_116 | transposase | - |
| EW996_RS23950 (92387) | 92387..92509 | - | 123 | WP_223200913.1 | Hok/Gef family protein | Toxin |
| EW996_RS24665 (92454) | 92454..92606 | - | 153 | Protein_118 | FlmC family protein | - |
| - (92594) | 92594..92799 | - | 206 | NuclAT_0 | - | Antitoxin |
| - (92594) | 92594..92799 | - | 206 | NuclAT_0 | - | Antitoxin |
| - (92594) | 92594..92799 | - | 206 | NuclAT_0 | - | Antitoxin |
| - (92594) | 92594..92799 | - | 206 | NuclAT_0 | - | Antitoxin |
| EW996_RS23955 (92756) | 92756..93070 | - | 315 | WP_074502941.1 | theronine dehydrogenase | - |
| EW996_RS23960 (93067) | 93067..93786 | - | 720 | WP_074502940.1 | plasmid SOS inhibition protein A | - |
| EW996_RS23965 (93783) | 93783..94205 | - | 423 | WP_074502939.1 | conjugation system SOS inhibitor PsiB | - |
| EW996_RS23970 (94260) | 94260..96212 | - | 1953 | WP_074502937.1 | ParB/RepB/Spo0J family partition protein | - |
| EW996_RS23975 (96271) | 96271..96504 | - | 234 | WP_044718095.1 | DUF905 domain-containing protein | - |
| EW996_RS23980 (96561) | 96561..97081 | - | 521 | Protein_124 | single-stranded DNA-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | csdD / csdC / csdA / csdB / estIa / etpA / etpB / eltB / eltA | 1..186206 | 186206 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 41 a.a. Molecular weight: 4538.38 Da Isoelectric Point: 8.2691
>T119015 WP_223200913.1 NZ_CP035883:c92509-92387 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK
Download Length: 123 bp
>T119015 NZ_CP035883:c92509-92387 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACTCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG
ATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACTCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG
Antitoxin
Download Length: 206 bp
>AT119015 NZ_CP035883:c92799-92594 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGTGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAAGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGTGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAAGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
