Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3116704..3117245 Replicon chromosome
Accession NZ_CP035882
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag EW996_RS15190 Protein ID WP_000615979.1
Coordinates 3116967..3117245 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A827BG71
Locus tag EW996_RS15185 Protein ID WP_000729710.1
Coordinates 3116704..3116964 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS15165 (3112387) 3112387..3113172 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
EW996_RS15170 (3113144) 3113144..3114856 + 1713 Protein_2982 flagellar biosynthesis protein FlhA -
EW996_RS15175 (3115072) 3115072..3115569 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
EW996_RS15180 (3115745) 3115745..3116518 - 774 WP_001353765.1 C40 family peptidase -
EW996_RS15185 (3116704) 3116704..3116964 + 261 WP_000729710.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EW996_RS15190 (3116967) 3116967..3117245 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EW996_RS15195 (3117401) 3117401..3118141 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EW996_RS15200 (3118112) 3118112..3118879 - 768 WP_000333379.1 class II glutamine amidotransferase -
EW996_RS15205 (3119085) 3119085..3119663 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T119005 WP_000615979.1 NZ_CP035882:3116967-3117245 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T119005 NZ_CP035882:3116967-3117245 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9365.69 Da        Isoelectric Point: 5.0758

>AT119005 WP_000729710.1 NZ_CP035882:3116704-3116964 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQSTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT119005 NZ_CP035882:3116704-3116964 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATCAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A827BG71

References