Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1186979..1187481 Replicon chromosome
Accession NZ_CP035882
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag EW996_RS05515 Protein ID WP_050491192.1
Coordinates 1187266..1187481 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EW996_RS05510 Protein ID WP_001259255.1
Coordinates 1186979..1187230 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS05485 (1182157) 1182157..1183224 - 1068 WP_000080081.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EW996_RS05490 (1183224) 1183224..1184294 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
EW996_RS05495 (1184291) 1184291..1185595 - 1305 WP_063116140.1 histidinol dehydrogenase -
EW996_RS05500 (1185601) 1185601..1186500 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EW996_RS05505 (1186646) 1186646..1186696 - 51 WP_001364200.1 his operon leader peptide -
EW996_RS05510 (1186979) 1186979..1187230 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EW996_RS05515 (1187266) 1187266..1187481 + 216 WP_050491192.1 Txe/YoeB family addiction module toxin Toxin
EW996_RS05520 (1187564) 1187564..1188388 + 825 WP_000754737.1 SDR family oxidoreductase -
EW996_RS05525 (1188434) 1188434..1189363 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
EW996_RS05530 (1189578) 1189578..1189640 + 63 WP_010723108.1 membrane protein YoeI -
EW996_RS05535 (1189630) 1189630..1190988 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EW996_RS05540 (1191167) 1191167..1192225 + 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
EW996_RS05545 (1192239) 1192239..1192466 + 228 WP_063116139.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(1-71)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8549.82 Da        Isoelectric Point: 9.8192

>T118989 WP_050491192.1 NZ_CP035882:1187266-1187481 [Escherichia coli]
MYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY

Download         Length: 216 bp

>T118989 NZ_CP035882:1187266-1187481 [Escherichia coli]
CTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGATCAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGA
AGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAGGTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGG
TATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGTCGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118989 WP_001259255.1 NZ_CP035882:1186979-1187230 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118989 NZ_CP035882:1186979-1187230 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References