Detailed information of TA system TA118960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 58354..58925
• Replicon: plasmid pB2C_96
• Accession: NZ_CP035876
• Organism: Escherichia coli strain B2C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EW998_RS24620
• Protein ID: WP_032323275.1
• Coordinates: 58644..58925 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EW998_RS24615
• Protein ID: WP_032323276.1
• Coordinates: 58354..58635 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW998_RS24580	53786..54577	-	792	WP_000532307.1	hypothetical protein	-
EW998_RS24585	54740..55042	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EW998_RS24590	55113..55451	+	339	WP_000806445.1	HigA family addiction module antitoxin	-
EW998_RS24595	55508..55867	+	360	WP_032323279.1	transcriptional regulator	-
EW998_RS24600	55931..56710	+	780	WP_032323278.1	hypothetical protein	-
EW998_RS24605	56746..57939	-	1194	WP_032323277.1	hypothetical protein	-
EW998_RS24610	57932..58261	-	330	WP_000542383.1	hypothetical protein	-
EW998_RS24615	58354..58635	-	282	WP_032323276.1	HigA family addiction module antitoxin	Antitoxin
EW998_RS24620	58644..58925	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EW998_RS24625	59262..59915	-	654	WP_023908597.1	hypothetical protein	-
EW998_RS24630	60310..60807	+	498	WP_001436217.1	hypothetical protein	-
EW998_RS26065	60828..61220	+	393	WP_000824759.1	hypothetical protein	-
EW998_RS24640	61217..61528	-	312	WP_000717724.1	hypothetical protein	-
EW998_RS24645	61819..62529	-	711	Protein_65	Rha family transcriptional regulator	-
EW998_RS26070	62529..62744	-	216	WP_000201263.1	host cell division inhibitor Icd-like protein	-
EW998_RS26075	62763..62882	-	120	WP_071526433.1	ash family protein	-
EW998_RS24655	63057..63224	-	168	WP_167895685.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96149	96149

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10606.14 Da
• Isoelectric Point: 10.2589

>T118960 WP_032323275.1 NZ_CP035876:c58925-58644 [Escherichia coli]
MIKSFRHKGLERFFKTGSTAGIQAKHAVKLRIQLTALNTAKRPDDMSAPGWELHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T118960 NZ_CP035876:c58925-58644 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACGCTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTGACTGCGCTTAATACAGCTAAACGCCCGGACGATATGTCTGCGCCTGGATGGGAATTGC
ACCCTCTAAAGGGGGCGGACCTTAAAGGTCACTGGGCAATATCGGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGAGAAGACGCAATTCTAGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10160.63 Da
• Isoelectric Point: 7.3179

>AT118960 WP_032323276.1 NZ_CP035876:c58635-58354 [Escherichia coli]
MSRMHNPAHPGAVLREYLGDMSVTDAAAALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TTRPNIRPLFFHA

Nucleotide

• Length: 282 bp

>AT118960 NZ_CP035876:c58635-58354 [Escherichia coli]
ATGAGTCGCATGCATAATCCCGCGCATCCAGGCGCGGTTCTTCGTGAATATTTGGGAGACATGTCAGTCACAGACGCCGC
TGCGGCATTAGGCATTACTCGCACGGCGCTTTCCCGAATTCTGAATGAAAAAGCAGGTATATCTGCTGATATGGCACTTC
GTCTGGAGTCCGCTCTTGGAACCAGTGCCGAAATGTGGACCGGTATGCAATCCCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCACTCGCCCAAACATCCGTCCATTATTTTTCCACGCATAA