Detailed information of TA system TA118959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 54740..55451
• Replicon: plasmid pB2C_96
• Accession: NZ_CP035876
• Organism: Escherichia coli strain B2C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: EW998_RS24585
• Protein ID: WP_000162415.1
• Coordinates: 54740..55042 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EW998_RS24590
• Protein ID: WP_000806445.1
• Coordinates: 55113..55451 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW998_RS24560	50423..51328	-	906	WP_032323280.1	recombination-associated protein RdgC	-
EW998_RS24565	51388..52041	-	654	WP_000828870.1	hypothetical protein	-
EW998_RS24570	52038..53018	-	981	WP_000046500.1	hypothetical protein	-
EW998_RS24575	53022..53789	-	768	WP_000203293.1	hypothetical protein	-
EW998_RS24580	53786..54577	-	792	WP_000532307.1	hypothetical protein	-
EW998_RS24585	54740..55042	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EW998_RS24590	55113..55451	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
EW998_RS24595	55508..55867	+	360	WP_032323279.1	transcriptional regulator	-
EW998_RS24600	55931..56710	+	780	WP_032323278.1	hypothetical protein	-
EW998_RS24605	56746..57939	-	1194	WP_032323277.1	hypothetical protein	-
EW998_RS24610	57932..58261	-	330	WP_000542383.1	hypothetical protein	-
EW998_RS24615	58354..58635	-	282	WP_032323276.1	HigA family addiction module antitoxin	-
EW998_RS24620	58644..58925	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EW998_RS24625	59262..59915	-	654	WP_023908597.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96149	96149

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T118959 WP_000162415.1 NZ_CP035876:54740-55042 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T118959 NZ_CP035876:54740-55042 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT118959 WP_000806445.1 NZ_CP035876:55113-55451 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT118959 NZ_CP035876:55113-55451 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3