Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4142210..4142904 | Replicon | chromosome |
| Accession | NZ_CP035874 | ||
| Organism | Escherichia coli strain B2C | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | EW998_RS20120 | Protein ID | WP_001263493.1 |
| Coordinates | 4142210..4142608 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | EW998_RS20125 | Protein ID | WP_000554757.1 |
| Coordinates | 4142611..4142904 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4137870) | 4137870..4137950 | - | 81 | NuclAT_12 | - | - |
| - (4137870) | 4137870..4137950 | - | 81 | NuclAT_12 | - | - |
| - (4137870) | 4137870..4137950 | - | 81 | NuclAT_12 | - | - |
| - (4137870) | 4137870..4137950 | - | 81 | NuclAT_12 | - | - |
| EW998_RS20090 (4137210) | 4137210..4138454 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| EW998_RS20095 (4138546) | 4138546..4139004 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| EW998_RS20100 (4139265) | 4139265..4140722 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| EW998_RS20105 (4140779) | 4140779..4141300 | - | 522 | Protein_3938 | peptide chain release factor H | - |
| EW998_RS20110 (4141299) | 4141299..4141502 | - | 204 | Protein_3939 | RtcB family protein | - |
| EW998_RS20115 (4141748) | 4141748..4142200 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| EW998_RS20120 (4142210) | 4142210..4142608 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| EW998_RS20125 (4142611) | 4142611..4142904 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| EW998_RS20130 (4142956) | 4142956..4144011 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| EW998_RS20135 (4144082) | 4144082..4145005 | - | 924 | WP_001232570.1 | putative lateral flagellar export/assembly protein LafU | - |
| EW998_RS20140 (4145008) | 4145008..4145871 | - | 864 | WP_001169518.1 | flagellar motor stator protein MotA | - |
| EW998_RS20145 (4145884) | 4145884..4146603 | - | 720 | WP_000938740.1 | FliA/WhiG family RNA polymerase sigma factor | - |
| EW998_RS20150 (4146623) | 4146623..4147090 | - | 468 | WP_000725257.1 | flagellar basal body-associated FliL family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T118955 WP_001263493.1 NZ_CP035874:c4142608-4142210 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T118955 NZ_CP035874:c4142608-4142210 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT118955 WP_000554757.1 NZ_CP035874:c4142904-4142611 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT118955 NZ_CP035874:c4142904-4142611 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|