Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 70477..70894 | Replicon | plasmid pE2528C1_95 |
| Accession | NZ_CP035872 | ||
| Organism | Escherichia coli strain E2528-C1 | ||
Toxin (Protein)
| Gene name | hok | Uniprot ID | G9G195 |
| Locus tag | EW999_RS24990 | Protein ID | WP_001323520.1 |
| Coordinates | 70778..70894 (+) | Length | 39 a.a. |
Antitoxin (RNA)
| Gene name | srnC | ||
| Locus tag | - | ||
| Coordinates | 70477..70671 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EW999_RS24970 (66675) | 66675..67235 | - | 561 | Protein_70 | IS3 family transposase | - |
| EW999_RS24975 (67297) | 67297..69260 | + | 1964 | Protein_71 | ParB/RepB/Spo0J family partition protein | - |
| EW999_RS24980 (69315) | 69315..69749 | + | 435 | WP_000845954.1 | conjugation system SOS inhibitor PsiB | - |
| EW999_RS24985 (69746) | 69746..70553 | + | 808 | Protein_73 | plasmid SOS inhibition protein A | - |
| - (70477) | 70477..70671 | + | 195 | NuclAT_0 | - | Antitoxin |
| - (70477) | 70477..70671 | + | 195 | NuclAT_0 | - | Antitoxin |
| - (70477) | 70477..70671 | + | 195 | NuclAT_0 | - | Antitoxin |
| - (70477) | 70477..70671 | + | 195 | NuclAT_0 | - | Antitoxin |
| EW999_RS25725 (70678) | 70678..70827 | + | 150 | Protein_74 | plasmid maintenance protein Mok | - |
| EW999_RS24990 (70778) | 70778..70894 | + | 117 | WP_001323520.1 | type I toxin-antitoxin system Hok family toxin | Toxin |
| EW999_RS24995 (71188) | 71188..72759 | - | 1572 | WP_099554548.1 | IS66 family transposase | - |
| EW999_RS25000 (72779) | 72779..73126 | - | 348 | WP_000624622.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EW999_RS25005 (73126) | 73126..73803 | - | 678 | WP_099554551.1 | IS66-like element accessory protein TnpA | - |
| EW999_RS25730 (73950) | 73950..74307 | + | 358 | Protein_79 | hypothetical protein | - |
| EW999_RS25015 (74357) | 74357..75034 | + | 678 | WP_001339397.1 | IS66-like element accessory protein TnpA | - |
| EW999_RS25020 (75034) | 75034..75381 | + | 348 | WP_000624622.1 | IS66 family insertion sequence element accessory protein TnpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | eltB / eltA / cssA | 1..95521 | 95521 | |
| - | inside | IScluster/Tn | - | - | 66675..90983 | 24308 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 39 a.a. Molecular weight: 4455.23 Da Isoelectric Point: 8.5110
>T118932 WP_001323520.1 NZ_CP035872:70778-70894 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK
Download Length: 117 bp
>T118932 NZ_CP035872:70778-70894 [Escherichia coli]
GTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGA
GGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG
GTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGA
GGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG
Antitoxin
Download Length: 195 bp
>AT118932 NZ_CP035872:70477-70671 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGTGCAGGGATATGAGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGTGCAGGGATATGAGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
