Detailed information of TA system TA118930

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41562..41789
• Replicon: plasmid pE2528C1_95
• Accession: NZ_CP035872
• Organism: Escherichia coli strain E2528-C1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: EW999_RS24830
• Protein ID: WP_064727294.1
• Coordinates: 41562..41684 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41733..41789 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW999_RS24805 (37173)	37173..37247	-	75	WP_001365705.1	RepA leader peptide Tap	-
EW999_RS24810 (37472)	37472..37732	-	261	WP_000083817.1	replication regulatory protein RepA	-
EW999_RS24815 (38256)	38256..39620	+	1365	Protein_42	IS21-like element ISEc12 family transposase	-
EW999_RS24825 (40931)	40931..41491	+	561	Protein_44	IS21-like element ISEc12 family helper ATPase IstB	-
EW999_RS24830 (41562)	41562..41684	-	123	WP_064727294.1	Hok/Gef family protein	Toxin
- (41733)	41733..41789	+	57	NuclAT_1	-	Antitoxin
- (41733)	41733..41789	+	57	NuclAT_1	-	Antitoxin
- (41733)	41733..41789	+	57	NuclAT_1	-	Antitoxin
- (41733)	41733..41789	+	57	NuclAT_1	-	Antitoxin
EW999_RS24835 (42033)	42033..42215	+	183	WP_001393357.1	hypothetical protein	-
EW999_RS24840 (42439)	42439..43170	-	732	WP_001734655.1	type-F conjugative transfer system pilin acetylase TraX	-
EW999_RS24845 (43167)	43167..43733	-	567	WP_024169751.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltB / eltA / cssA	1..95521	95521

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-36)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4495.51 Da
• Isoelectric Point: 8.1317

>T118930 WP_064727294.1 NZ_CP035872:c41684-41562 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNCVLP

Nucleotide

• Length: 123 bp

>T118930 NZ_CP035872:c41684-41562 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGAGGAAATTGCGTATTACCGTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT118930 NZ_CP035872:41733-41789 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin