Detailed information of TA system TA118901

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1736..2148
• Replicon: plasmid pE275a_100
• Accession: NZ_CP035867
• Organism: Escherichia coli strain E275 a

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: EXE34_RS25325
• Protein ID: WP_223200913.1
• Coordinates: 1736..1858 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	1963..2148 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE34_RS25320 (866)	866..1387	-	522	Protein_1	IS66-like element accessory protein TnpA	-
EXE34_RS25325 (1736)	1736..1858	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
EXE34_RS26760 (1803)	1803..1955	-	153	Protein_3	FlmC family protein	-
- (1963)	1963..2148	-	186	NuclAT_0	-	Antitoxin
- (1963)	1963..2148	-	186	NuclAT_0	-	Antitoxin
- (1963)	1963..2148	-	186	NuclAT_0	-	Antitoxin
- (1963)	1963..2148	-	186	NuclAT_0	-	Antitoxin
EXE34_RS25330 (2105)	2105..2419	-	315	WP_000872086.1	hypothetical protein	-
EXE34_RS25335 (2416)	2416..3135	-	720	WP_000994093.1	plasmid SOS inhibition protein A	-
EXE34_RS25340 (3132)	3132..3566	-	435	WP_074526032.1	conjugation system SOS inhibitor PsiB	-
EXE34_RS25345 (3621)	3621..5588	-	1968	WP_216285026.1	ParB/RepB/Spo0J family partition protein	-
EXE34_RS25350 (5649)	5649..5882	-	234	WP_000005973.1	DUF905 domain-containing protein	-
EXE34_RS25355 (5939)	5939..6472	-	534	WP_080849558.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpA / etpB	1..100141	100141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T118901 WP_223200913.1 NZ_CP035867:c1858-1736 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T118901 NZ_CP035867:c1858-1736 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 186 bp

>AT118901 NZ_CP035867:c2148-1963 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTCATTAACCCACGAGGC

Structures

Antitoxin