Detailed information of TA system TA118880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 682682..683376
• Replicon: chromosome
• Accession: NZ_CP035865
• Organism: Escherichia coli strain E275 a

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: EXE34_RS03220
• Protein ID: WP_001263493.1
• Coordinates: 682978..683376 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: EXE34_RS03215
• Protein ID: WP_000554757.1
• Coordinates: 682682..682975 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE34_RS03195 (678314)	678314..678811	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
EXE34_RS03200 (679035)	679035..680747	-	1713	Protein_629	flagellar biosynthesis protein FlhA	-
EXE34_RS03205 (680719)	680719..681504	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
EXE34_RS03210 (681575)	681575..682630	+	1056	WP_085452615.1	DNA polymerase IV	-
EXE34_RS03215 (682682)	682682..682975	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EXE34_RS03220 (682978)	682978..683376	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EXE34_RS03225 (683386)	683386..683838	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
EXE34_RS03230 (684084)	684084..684287	+	204	Protein_635	RtcB family protein	-
EXE34_RS03235 (684286)	684286..684807	+	522	Protein_636	peptide chain release factor H	-
EXE34_RS03240 (684864)	684864..686321	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
EXE34_RS03245 (686582)	686582..687040	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (687636)	687636..687716	+	81	NuclAT_11	-	-
- (687636)	687636..687716	+	81	NuclAT_11	-	-
- (687636)	687636..687716	+	81	NuclAT_11	-	-
- (687636)	687636..687716	+	81	NuclAT_11	-	-
EXE34_RS03250 (687132)	687132..688376	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T118880 WP_001263493.1 NZ_CP035865:682978-683376 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T118880 NZ_CP035865:682978-683376 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT118880 WP_000554757.1 NZ_CP035865:682682-682975 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT118880 NZ_CP035865:682682-682975 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6