Detailed information of TA system TA118816

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 151841..152095
• Replicon: plasmid pTW10590_155
• Accession: NZ_CP035851
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EXE32_RS24815
• Protein ID: WP_001312851.1
• Coordinates: 151841..151990 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	152034..152095 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS24790 (147083)	147083..148264	-	1182	WP_126688001.1	DUF3800 domain-containing protein	-
EXE32_RS26775 (148452)	148452..148535	+	84	Protein_183	hypothetical protein	-
EXE32_RS24795 (148522)	148522..149109	+	588	Protein_184	IS91 family transposase	-
EXE32_RS26780 (149259)	149259..149438	-	180	WP_126688003.1	hypothetical protein	-
EXE32_RS24800 (150142)	150142..150999	-	858	WP_216349611.1	incFII family plasmid replication initiator RepA	-
EXE32_RS24805 (150992)	150992..151066	-	75	WP_011387702.1	RepA leader peptide Tap	-
EXE32_RS24810 (151300)	151300..151557	-	258	WP_000083826.1	replication regulatory protein RepA	-
EXE32_RS24815 (151841)	151841..151990	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (152034)	152034..152095	+	62	NuclAT_1	-	Antitoxin
- (152034)	152034..152095	+	62	NuclAT_1	-	Antitoxin
- (152034)	152034..152095	+	62	NuclAT_1	-	Antitoxin
- (152034)	152034..152095	+	62	NuclAT_1	-	Antitoxin
EXE32_RS24820 (152247)	152247..152711	-	465	WP_001756974.1	hypothetical protein	-
EXE32_RS24825 (152946)	152946..154118	+	1173	WP_000952428.1	IS21-like element ISEc62 family transposase	-
EXE32_RS24830 (154118)	154118..154912	+	795	WP_000544809.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8	cofA	1..155153	155153
	-	inside	IScluster/Tn	-	-	144672..154912	10240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T118816 WP_001312851.1 NZ_CP035851:c151990-151841 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T118816 NZ_CP035851:c151990-151841 [Escherichia coli]
ATGACGAAATATGCCCTTATAGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT118816 NZ_CP035851:152034-152095 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin