Detailed information of TA system TA118813

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29940..30353
• Replicon: plasmid pTW10590_155
• Accession: NZ_CP035851
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: EXE32_RS24095
• Protein ID: WP_223163518.1
• Coordinates: 29940..30062 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	30154..30353 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS26725 (25436)	25436..25609	+	174	Protein_31	hypothetical protein	-
EXE32_RS24075 (26338)	26338..28005	+	1668	WP_001091724.1	group II intron reverse transcriptase/maturase	-
EXE32_RS24080 (28470)	28470..28712	-	243	WP_000540588.1	hypothetical protein	-
EXE32_RS24085 (28771)	28771..29094	-	324	WP_000533253.1	hypothetical protein	-
EXE32_RS24090 (29390)	29390..29639	+	250	Protein_35	hypothetical protein	-
EXE32_RS24095 (29940)	29940..30062	-	123	WP_223163518.1	Hok/Gef family protein	Toxin
EXE32_RS26730 (30007)	30007..30120	-	114	Protein_37	DUF5431 family protein	-
- (30154)	30154..30353	-	200	NuclAT_0	-	Antitoxin
- (30154)	30154..30353	-	200	NuclAT_0	-	Antitoxin
- (30154)	30154..30353	-	200	NuclAT_0	-	Antitoxin
- (30154)	30154..30353	-	200	NuclAT_0	-	Antitoxin
EXE32_RS24100 (30310)	30310..30624	-	315	WP_000870669.1	hypothetical protein	-
EXE32_RS24105 (30621)	30621..31340	-	720	WP_000994097.1	plasmid SOS inhibition protein A	-
EXE32_RS24110 (31337)	31337..31771	-	435	WP_000845966.1	conjugation system SOS inhibitor PsiB	-
EXE32_RS24115 (31826)	31826..33784	-	1959	WP_001145496.1	ParB/RepB/Spo0J family partition protein	-
EXE32_RS24120 (33843)	33843..34076	-	234	WP_000005975.1	DUF905 domain-containing protein	-
EXE32_RS24125 (34132)	34132..34659	-	528	WP_000290816.1	single-stranded DNA-binding protein	-
EXE32_RS26735 (34961)	34961..35134	+	174	Protein_44	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8	cofA	1..155153	155153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4506.32 Da
• Isoelectric Point: 8.2691

>T118813 WP_223163518.1 NZ_CP035851:c30062-29940 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESGGK

Nucleotide

• Length: 123 bp

>T118813 NZ_CP035851:c30062-29940 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAGGTTACGGCAAGTTTGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 200 bp

>AT118813 NZ_CP035851:c30353-30154 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin