Detailed information of TA system TA118802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3162737..3163239
• Replicon: chromosome
• Accession: NZ_CP035850
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EXE32_RS15110
• Protein ID: WP_000767835.1
• Coordinates: 3162985..3163239 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: EXE32_RS15105
• Protein ID: WP_001259255.1
• Coordinates: 3162737..3162988 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS15080 (3157914)	3157914..3158981	-	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
EXE32_RS15085 (3158981)	3158981..3160051	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
EXE32_RS15090 (3160048)	3160048..3161352	-	1305	WP_001413482.1	histidinol dehydrogenase	-
EXE32_RS15095 (3161358)	3161358..3162257	-	900	WP_000131774.1	ATP phosphoribosyltransferase	-
EXE32_RS15100 (3162403)	3162403..3162453	-	51	WP_071593552.1	his operon leader peptide	-
EXE32_RS15105 (3162737)	3162737..3162988	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
EXE32_RS15110 (3162985)	3162985..3163239	+	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
EXE32_RS15115 (3163322)	3163322..3164146	+	825	WP_000754737.1	SDR family oxidoreductase	-
EXE32_RS15120 (3164192)	3164192..3165121	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
EXE32_RS15125 (3165336)	3165336..3165398	+	63	WP_010723108.1	membrane protein YoeI	-
EXE32_RS15130 (3165388)	3165388..3166746	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
EXE32_RS15135 (3166925)	3166925..3167983	+	1059	WP_000492320.1	thiosulfate utilization transporter TsuA/YeeE	-
EXE32_RS15140 (3167997)	3167997..3168224	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	wza / gmd / wcaG / manB / manB / wcaJ / galF / gmd / wcaG / manB / gnd / ugd	2975052..3196050	220998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T118802 WP_000767835.1 NZ_CP035850:3162985-3163239 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T118802 NZ_CP035850:3162985-3163239 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT118802 WP_001259255.1 NZ_CP035850:3162737-3162988 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT118802 NZ_CP035850:3162737-3162988 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0