Detailed information of TA system TA118796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1139732..1140334
• Replicon: chromosome
• Accession: NZ_CP035850
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EXE32_RS05345
• Protein ID: WP_000897305.1
• Coordinates: 1140023..1140334 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXE32_RS05340
• Protein ID: WP_000356397.1
• Coordinates: 1139732..1140022 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS05315 (1135279)	1135279..1135914	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EXE32_RS05320 (1135911)	1135911..1136840	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EXE32_RS05325 (1137239)	1137239..1138219	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
EXE32_RS05330 (1138448)	1138448..1138690	-	243	WP_001086388.1	protein YiiF	-
EXE32_RS05335 (1138909)	1138909..1139127	-	219	WP_001295676.1	CopG family transcriptional regulator	-
EXE32_RS05340 (1139732)	1139732..1140022	-	291	WP_000356397.1	NadS family protein	Antitoxin
EXE32_RS05345 (1140023)	1140023..1140334	-	312	WP_000897305.1	hypothetical protein	Toxin
EXE32_RS05350 (1140563)	1140563..1141471	+	909	WP_001162704.1	alpha/beta hydrolase	-
EXE32_RS05355 (1141535)	1141535..1142476	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
EXE32_RS05360 (1142521)	1142521..1142958	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EXE32_RS05365 (1142955)	1142955..1143827	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EXE32_RS05370 (1143821)	1143821..1144420	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1137239..1138219	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118796 WP_000897305.1 NZ_CP035850:c1140334-1140023 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118796 NZ_CP035850:c1140334-1140023 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118796 WP_000356397.1 NZ_CP035850:c1140022-1139732 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118796 NZ_CP035850:c1140022-1139732 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7