Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 259401..259942 Replicon chromosome
Accession NZ_CP035850
Organism Escherichia coli strain TW10590

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EXE32_RS01240 Protein ID WP_000615976.1
Coordinates 259664..259942 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EXE32_RS01235 Protein ID WP_000729704.1
Coordinates 259401..259661 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE32_RS01210 (255184) 255184..255969 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
EXE32_RS01215 (255941) 255941..257653 + 1713 Protein_242 flagellar biosynthesis protein FlhA -
EXE32_RS01220 (257758) 257758..258036 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EXE32_RS01225 (258029) 258029..258385 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EXE32_RS01230 (258442) 258442..259215 - 774 WP_000543897.1 C40 family peptidase -
EXE32_RS01235 (259401) 259401..259661 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EXE32_RS01240 (259664) 259664..259942 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EXE32_RS01245 (260098) 260098..260838 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EXE32_RS01250 (260809) 260809..261576 - 768 WP_000333380.1 class II glutamine amidotransferase -
EXE32_RS01255 (261782) 261782..262360 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T118792 WP_000615976.1 NZ_CP035850:259664-259942 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118792 NZ_CP035850:259664-259942 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT118792 WP_000729704.1 NZ_CP035850:259401-259661 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118792 NZ_CP035850:259401-259661 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References