Detailed information of TA system TA118763

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 18357..18769
• Replicon: plasmid pTW10722_93
• Accession: NZ_CP035844
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: EXA02_RS27505
• Protein ID: WP_223195199.1
• Coordinates: 18647..18769 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	18357..18542 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS27475 (14038)	14038..14565	+	528	WP_000290805.1	single-stranded DNA-binding protein	-
EXA02_RS27480 (14622)	14622..14855	+	234	WP_000005968.1	DUF905 domain-containing protein	-
EXA02_RS27485 (14917)	14917..16884	+	1968	WP_000117113.1	ParB/RepB/Spo0J family partition protein	-
EXA02_RS27490 (16939)	16939..17373	+	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
EXA02_RS27495 (17370)	17370..18089	+	720	WP_000994093.1	plasmid SOS inhibition protein A	-
EXA02_RS27500 (18086)	18086..18400	+	315	WP_000872086.1	hypothetical protein	-
- (18357)	18357..18542	+	186	NuclAT_0	-	Antitoxin
- (18357)	18357..18542	+	186	NuclAT_0	-	Antitoxin
- (18357)	18357..18542	+	186	NuclAT_0	-	Antitoxin
- (18357)	18357..18542	+	186	NuclAT_0	-	Antitoxin
EXA02_RS28565 (18550)	18550..18702	+	153	Protein_28	FlmC family protein	-
EXA02_RS27505 (18647)	18647..18769	+	123	WP_223195199.1	Hok/Gef family protein	Toxin
EXA02_RS27510 (19007)	19007..19385	-	379	Protein_30	hypothetical protein	-
EXA02_RS27515 (19686)	19686..19967	+	282	WP_000107523.1	hypothetical protein	-
EXA02_RS27520 (20009)	20009..20251	+	243	WP_000540593.1	hypothetical protein	-
EXA02_RS27525 (20592)	20592..20807	+	216	WP_024179973.1	hypothetical protein	-
EXA02_RS27530 (20968)	20968..21129	-	162	Protein_34	IS3 family transposase	-
EXA02_RS27535 (21265)	21265..21717	-	453	WP_000163902.1	transglycosylase SLT domain-containing protein	-
EXA02_RS27540 (22189)	22189..22575	+	387	WP_001137405.1	conjugal transfer relaxosome DNA-binding protein TraM	-
EXA02_RS27545 (23054)	23054..23335	+	282	WP_000974580.1	hypothetical protein	-
EXA02_RS27550 (23551)	23551..23718	+	168	WP_157187929.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / sul1 / qacE / ant(3'')-Ia / dfrA15	-	1..93485	93485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T118763 WP_223195199.1 NZ_CP035844:18647-18769 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T118763 NZ_CP035844:18647-18769 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 186 bp

>AT118763 NZ_CP035844:18357-18542 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTCATTAACCCACGAGGC

Structures

Antitoxin