Detailed information of TA system TA118739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2222880..2223482
• Replicon: chromosome
• Accession: NZ_CP035841
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EXA02_RS10865
• Protein ID: WP_000897305.1
• Coordinates: 2223171..2223482 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXA02_RS10860
• Protein ID: WP_000356397.1
• Coordinates: 2222880..2223170 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS10835 (2218806)	2218806..2219708	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EXA02_RS10840 (2219705)	2219705..2220340	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EXA02_RS10845 (2220337)	2220337..2221266	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EXA02_RS10850 (2221596)	2221596..2221838	-	243	WP_001086388.1	protein YiiF	-
EXA02_RS10855 (2222057)	2222057..2222275	-	219	WP_001295676.1	CopG family transcriptional regulator	-
EXA02_RS10860 (2222880)	2222880..2223170	-	291	WP_000356397.1	NadS family protein	Antitoxin
EXA02_RS10865 (2223171)	2223171..2223482	-	312	WP_000897305.1	hypothetical protein	Toxin
EXA02_RS10870 (2223711)	2223711..2224619	+	909	WP_001162704.1	alpha/beta hydrolase	-
EXA02_RS10875 (2224683)	2224683..2225624	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EXA02_RS10880 (2225669)	2225669..2226106	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EXA02_RS10885 (2226103)	2226103..2226975	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EXA02_RS10890 (2226969)	2226969..2227568	-	600	WP_001295269.1	glucose-1-phosphatase	-
EXA02_RS10895 (2227667)	2227667..2228452	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118739 WP_000897305.1 NZ_CP035841:c2223482-2223171 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118739 NZ_CP035841:c2223482-2223171 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118739 WP_000356397.1 NZ_CP035841:c2223170-2222880 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118739 NZ_CP035841:c2223170-2222880 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7