Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1241716..1242257 Replicon chromosome
Accession NZ_CP035841
Organism Escherichia coli strain TW10722

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EXA02_RS06235 Protein ID WP_000615976.1
Coordinates 1241979..1242257 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EXA02_RS06230 Protein ID WP_000729704.1
Coordinates 1241716..1241976 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA02_RS06205 (1237499) 1237499..1238284 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
EXA02_RS06210 (1238256) 1238256..1239968 + 1713 Protein_1212 flagellar biosynthesis protein FlhA -
EXA02_RS06215 (1240073) 1240073..1240351 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EXA02_RS06220 (1240344) 1240344..1240700 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EXA02_RS06225 (1240757) 1240757..1241530 - 774 Protein_1215 C40 family peptidase -
EXA02_RS06230 (1241716) 1241716..1241976 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EXA02_RS06235 (1241979) 1241979..1242257 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EXA02_RS06240 (1242413) 1242413..1243153 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EXA02_RS06245 (1243124) 1243124..1243891 - 768 WP_000333380.1 class II glutamine amidotransferase -
EXA02_RS06250 (1244097) 1244097..1244675 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T118732 WP_000615976.1 NZ_CP035841:1241979-1242257 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118732 NZ_CP035841:1241979-1242257 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT118732 WP_000729704.1 NZ_CP035841:1241716-1241976 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118732 NZ_CP035841:1241716-1241976 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References