Detailed information of TA system TA118728

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 210082..210307
• Replicon: chromosome
• Accession: NZ_CP035841
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: EXA02_RS01195
• Protein ID: WP_000813254.1
• Coordinates: 210082..210237 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	210249..210307 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS01155	205890..206087	-	198	WP_000917767.1	hypothetical protein	-
EXA02_RS01160	206400..206942	-	543	WP_000640042.1	DUF1133 family protein	-
EXA02_RS01165	206951..207316	-	366	WP_000140022.1	RusA family crossover junction endodeoxyribonuclease	-
EXA02_RS01170	207317..208375	-	1059	WP_001265297.1	DUF968 domain-containing protein	-
EXA02_RS01175	208377..208655	-	279	WP_023607105.1	hypothetical protein	-
EXA02_RS01180	208722..208982	-	261	WP_000701918.1	hypothetical protein	-
EXA02_RS01185	209183..209668	-	486	WP_000818165.1	type II toxin-antitoxin system death-on-curing family toxin	-
EXA02_RS01190	209687..209866	-	180	WP_001277775.1	hypothetical protein	-
EXA02_RS01195	210082..210237	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	210249..210307	+	59	-	-	Antitoxin
EXA02_RS01200	210529..210951	-	423	WP_000160146.1	hypothetical protein	-
EXA02_RS01205	211191..211472	-	282	Protein_230	DUF4406 domain-containing protein	-
EXA02_RS01210	211469..211750	-	282	WP_001509842.1	hypothetical protein	-
EXA02_RS01215	211783..212544	-	762	WP_000450703.1	DUF1627 domain-containing protein	-
EXA02_RS01220	212566..213312	-	747	WP_000788772.1	ATP-binding protein	-
EXA02_RS01225	213318..214283	-	966	WP_000054517.1	hypothetical protein	-
EXA02_RS01230	214264..214785	-	522	WP_000705382.1	toxin YdaT family protein	-
EXA02_RS01235	214769..214996	-	228	WP_000476985.1	Cro/CI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	176778..228938	52160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T118728 WP_000813254.1 NZ_CP035841:c210237-210082 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T118728 NZ_CP035841:c210237-210082 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT118728 NZ_CP035841:210249-210307 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin