Detailed information of TA system TA118723

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30269..30533
• Replicon: plasmid pWS1933D_74
• Accession: NZ_CP035840
• Organism: Escherichia coli strain WS1933D

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: EXA03_RS26235
• Protein ID: WP_001303307.1
• Coordinates: 30269..30421 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	30471..30533 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA03_RS26205 (25525)	25525..27693	+	2169	WP_000698356.1	DotA/TraY family protein	-
EXA03_RS26210 (27764)	27764..28426	+	663	WP_000644795.1	plasmid IncI1-type surface exclusion protein ExcA	-
EXA03_RS26215 (28498)	28498..28707	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
EXA03_RS26220 (29109)	29109..29285	+	177	WP_001054898.1	hypothetical protein	-
EXA03_RS26225 (29350)	29350..29646	-	297	WP_001275298.1	DinQ-like type I toxin DqlB	-
EXA03_RS26230 (29946)	29946..30197	+	252	WP_001291965.1	hypothetical protein	-
EXA03_RS26235 (30269)	30269..30421	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
- (30471)	30471..30533	+	63	NuclAT_0	-	Antitoxin
- (30471)	30471..30533	+	63	NuclAT_0	-	Antitoxin
- (30471)	30471..30533	+	63	NuclAT_0	-	Antitoxin
- (30471)	30471..30533	+	63	NuclAT_0	-	Antitoxin
EXA03_RS26240 (30742)	30742..31827	+	1086	WP_000080544.1	hypothetical protein	-
- (31897)	31897..31955	+	59	NuclAT_1	-	-
- (31897)	31897..31955	+	59	NuclAT_1	-	-
- (31897)	31897..31955	+	59	NuclAT_1	-	-
- (31897)	31897..31955	+	59	NuclAT_1	-	-
EXA03_RS26245 (32135)	32135..33343	+	1209	WP_001295719.1	IncI1-type conjugal transfer protein TrbA	-
EXA03_RS26250 (33362)	33362..34432	+	1071	WP_000151590.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..74294	74294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T118723 WP_001303307.1 NZ_CP035840:c30421-30269 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T118723 NZ_CP035840:c30421-30269 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT118723 NZ_CP035840:30471-30533 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin