Detailed information of TA system TA118711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1290403..1290905
• Replicon: chromosome
• Accession: NZ_CP035836
• Organism: Escherichia coli strain WS1933D

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: EXA03_RS06585
• Protein ID: WP_000767829.1
• Coordinates: 1290403..1290657 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: EXA03_RS06590
• Protein ID: WP_001259255.1
• Coordinates: 1290654..1290905 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA03_RS06555 (1285418)	1285418..1285645	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
EXA03_RS06560 (1285659)	1285659..1286717	-	1059	WP_000492323.1	thiosulfate utilization transporter TsuA/YeeE	-
EXA03_RS06565 (1286896)	1286896..1288254	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
EXA03_RS06570 (1288244)	1288244..1288306	-	63	WP_010723108.1	membrane protein YoeI	-
EXA03_RS06575 (1288521)	1288521..1289450	-	930	WP_000803341.1	LysR substrate-binding domain-containing protein	-
EXA03_RS06580 (1289496)	1289496..1290320	-	825	WP_000754755.1	SDR family oxidoreductase	-
EXA03_RS06585 (1290403)	1290403..1290657	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
EXA03_RS06590 (1290654)	1290654..1290905	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
EXA03_RS06595 (1291189)	1291189..1291239	+	51	WP_001364200.1	his operon leader peptide	-
EXA03_RS06600 (1291385)	1291385..1292284	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
EXA03_RS06605 (1292290)	1292290..1293594	+	1305	WP_001484680.1	histidinol dehydrogenase	-
EXA03_RS06610 (1293591)	1293591..1294661	+	1071	WP_001485892.1	histidinol-phosphate transaminase	-
EXA03_RS06615 (1294661)	1294661..1295728	+	1068	WP_000080097.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T118711 WP_000767829.1 NZ_CP035836:c1290657-1290403 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T118711 NZ_CP035836:c1290657-1290403 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT118711 WP_001259255.1 NZ_CP035836:c1290905-1290654 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT118711 NZ_CP035836:c1290905-1290654 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0