Detailed information of TA system TA118698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4930695..4931297
• Replicon: chromosome
• Accession: NZ_CP035832
• Organism: Escherichia coli strain 025542-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EXA04_RS23920
• Protein ID: WP_000897305.1
• Coordinates: 4930986..4931297 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXA04_RS23915
• Protein ID: WP_000356397.1
• Coordinates: 4930695..4930985 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA04_RS23890 (4926621)	4926621..4927523	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EXA04_RS23895 (4927520)	4927520..4928155	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EXA04_RS23900 (4928152)	4928152..4929081	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EXA04_RS23905 (4929411)	4929411..4929653	-	243	WP_001086388.1	protein YiiF	-
EXA04_RS23910 (4929872)	4929872..4930090	-	219	WP_001295676.1	CopG family transcriptional regulator	-
EXA04_RS23915 (4930695)	4930695..4930985	-	291	WP_000356397.1	NadS family protein	Antitoxin
EXA04_RS23920 (4930986)	4930986..4931297	-	312	WP_000897305.1	hypothetical protein	Toxin
EXA04_RS23925 (4931526)	4931526..4932434	+	909	WP_001162704.1	alpha/beta hydrolase	-
EXA04_RS23930 (4932498)	4932498..4933439	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EXA04_RS23935 (4933484)	4933484..4933921	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EXA04_RS23940 (4933918)	4933918..4934790	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EXA04_RS23945 (4934784)	4934784..4935383	-	600	WP_001315111.1	glucose-1-phosphatase	-
EXA04_RS23950 (4935482)	4935482..4936267	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118698 WP_000897305.1 NZ_CP035832:c4931297-4930986 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118698 NZ_CP035832:c4931297-4930986 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118698 WP_000356397.1 NZ_CP035832:c4930985-4930695 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118698 NZ_CP035832:c4930985-4930695 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7