Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4093784..4094325 Replicon chromosome
Accession NZ_CP035832
Organism Escherichia coli strain 025542-1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EXA04_RS20100 Protein ID WP_000615976.1
Coordinates 4094047..4094325 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EXA04_RS20095 Protein ID WP_000729704.1
Coordinates 4093784..4094044 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA04_RS20070 (4089567) 4089567..4090352 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
EXA04_RS20075 (4090324) 4090324..4092036 + 1713 Protein_3938 flagellar biosynthesis protein FlhA -
EXA04_RS20080 (4092141) 4092141..4092419 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EXA04_RS20085 (4092412) 4092412..4092768 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EXA04_RS20090 (4092825) 4092825..4093598 - 774 WP_122996850.1 C40 family peptidase -
EXA04_RS20095 (4093784) 4093784..4094044 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EXA04_RS20100 (4094047) 4094047..4094325 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EXA04_RS20105 (4094481) 4094481..4095221 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EXA04_RS20110 (4095192) 4095192..4095959 - 768 WP_000333380.1 class II glutamine amidotransferase -
EXA04_RS20115 (4096165) 4096165..4096743 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T118695 WP_000615976.1 NZ_CP035832:4094047-4094325 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118695 NZ_CP035832:4094047-4094325 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT118695 WP_000729704.1 NZ_CP035832:4093784-4094044 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118695 NZ_CP035832:4093784-4094044 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References