Detailed information of TA system TA118681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1964678..1965180
• Replicon: chromosome
• Accession: NZ_CP035832
• Organism: Escherichia coli strain 025542-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EXA04_RS09450
• Protein ID: WP_000767835.1
• Coordinates: 1964926..1965180 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: EXA04_RS09445
• Protein ID: WP_001259255.1
• Coordinates: 1964678..1964929 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA04_RS09420 (1959855)	1959855..1960922	-	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
EXA04_RS09425 (1960922)	1960922..1961992	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
EXA04_RS09430 (1961989)	1961989..1963293	-	1305	WP_001356284.1	histidinol dehydrogenase	-
EXA04_RS09435 (1963299)	1963299..1964198	-	900	WP_000131774.1	ATP phosphoribosyltransferase	-
EXA04_RS09440 (1964344)	1964344..1964394	-	51	WP_001364200.1	his operon leader peptide	-
EXA04_RS09445 (1964678)	1964678..1964929	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
EXA04_RS09450 (1964926)	1964926..1965180	+	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
EXA04_RS09455 (1965263)	1965263..1966087	+	825	WP_000754737.1	SDR family oxidoreductase	-
EXA04_RS09460 (1966133)	1966133..1967062	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
EXA04_RS09465 (1967277)	1967277..1967339	+	63	WP_010723108.1	membrane protein YoeI	-
EXA04_RS09470 (1967329)	1967329..1968687	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
EXA04_RS09475 (1968866)	1968866..1969924	+	1059	WP_000492320.1	thiosulfate utilization transporter TsuA/YeeE	-
EXA04_RS09480 (1969938)	1969938..1970165	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T118681 WP_000767835.1 NZ_CP035832:1964926-1965180 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T118681 NZ_CP035832:1964926-1965180 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT118681 WP_001259255.1 NZ_CP035832:1964678-1964929 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT118681 NZ_CP035832:1964678-1964929 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0