Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4547591..4548285 | Replicon | chromosome |
| Accession | NZ_CP035822 | ||
| Organism | Escherichia coli strain WS4437A-1 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | EXA06_RS22015 | Protein ID | WP_001263489.1 |
| Coordinates | 4547887..4548285 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | - |
| Locus tag | EXA06_RS22010 | Protein ID | WP_001412607.1 |
| Coordinates | 4547591..4547884 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EXA06_RS21985 (4543408) | 4543408..4543875 | + | 468 | WP_000725257.1 | flagellar basal body-associated FliL family protein | - |
| EXA06_RS21990 (4543895) | 4543895..4544611 | + | 717 | WP_000938723.1 | FliA/WhiG family RNA polymerase sigma factor | - |
| EXA06_RS21995 (4544624) | 4544624..4545487 | + | 864 | WP_001169518.1 | flagellar motor stator protein MotA | - |
| EXA06_RS22000 (4545490) | 4545490..4546413 | + | 924 | WP_001232546.1 | putative lateral flagellar export/assembly protein LafU | - |
| EXA06_RS22005 (4546484) | 4546484..4547539 | + | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| EXA06_RS22010 (4547591) | 4547591..4547884 | + | 294 | WP_001412607.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| EXA06_RS22015 (4547887) | 4547887..4548285 | + | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| EXA06_RS22020 (4548295) | 4548295..4548747 | + | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| EXA06_RS22025 (4549065) | 4549065..4549271 | + | 207 | Protein_4310 | RtcB family protein | - |
| EXA06_RS22030 (4549267) | 4549267..4549788 | + | 522 | Protein_4311 | peptide chain release factor H | - |
| EXA06_RS22035 (4549845) | 4549845..4551302 | - | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| EXA06_RS22040 (4551563) | 4551563..4552021 | + | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| - (4552617) | 4552617..4552697 | + | 81 | NuclAT_11 | - | - |
| - (4552617) | 4552617..4552697 | + | 81 | NuclAT_11 | - | - |
| - (4552617) | 4552617..4552697 | + | 81 | NuclAT_11 | - | - |
| - (4552617) | 4552617..4552697 | + | 81 | NuclAT_11 | - | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T118643 WP_001263489.1 NZ_CP035822:4547887-4548285 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T118643 NZ_CP035822:4547887-4548285 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11199.75 Da Isoelectric Point: 5.2170
>AT118643 WP_001412607.1 NZ_CP035822:4547591-4547884 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDLKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDLKE
Download Length: 294 bp
>AT118643 NZ_CP035822:4547591-4547884 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACCTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACCTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|