Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3765425..3765966 Replicon chromosome
Accession NZ_CP035819
Organism Escherichia coli strain WS3294A

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EXA07_RS18050 Protein ID WP_000615976.1
Coordinates 3765425..3765703 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EXA07_RS18055 Protein ID WP_000729704.1
Coordinates 3765706..3765966 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA07_RS18035 (3763007) 3763007..3763585 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EXA07_RS18040 (3763791) 3763791..3764558 + 768 WP_000333380.1 class II glutamine amidotransferase -
EXA07_RS18045 (3764529) 3764529..3765269 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EXA07_RS18050 (3765425) 3765425..3765703 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EXA07_RS18055 (3765706) 3765706..3765966 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EXA07_RS18060 (3766152) 3766152..3766925 + 774 WP_000543895.1 C40 family peptidase -
EXA07_RS18065 (3766982) 3766982..3767338 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
EXA07_RS18070 (3767331) 3767331..3767609 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EXA07_RS18075 (3767714) 3767714..3769426 - 1713 Protein_3523 flagellar biosynthesis protein FlhA -
EXA07_RS18080 (3769398) 3769398..3770183 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3756476..3767609 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T118617 WP_000615976.1 NZ_CP035819:c3765703-3765425 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118617 NZ_CP035819:c3765703-3765425 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT118617 WP_000729704.1 NZ_CP035819:c3765966-3765706 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118617 NZ_CP035819:c3765966-3765706 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References