Detailed information of TA system TA118614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2927605..2928207
• Replicon: chromosome
• Accession: NZ_CP035819
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EXA07_RS14195
• Protein ID: WP_000897305.1
• Coordinates: 2927605..2927916 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXA07_RS14200
• Protein ID: WP_000356397.1
• Coordinates: 2927917..2928207 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS14170 (2923519)	2923519..2924118	+	600	WP_001295269.1	glucose-1-phosphatase	-
EXA07_RS14175 (2924112)	2924112..2924984	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EXA07_RS14180 (2924981)	2924981..2925418	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EXA07_RS14185 (2925463)	2925463..2926404	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EXA07_RS14190 (2926468)	2926468..2927376	-	909	WP_001162704.1	alpha/beta hydrolase	-
EXA07_RS14195 (2927605)	2927605..2927916	+	312	WP_000897305.1	hypothetical protein	Toxin
EXA07_RS14200 (2927917)	2927917..2928207	+	291	WP_000356397.1	NadS family protein	Antitoxin
EXA07_RS14205 (2928812)	2928812..2929030	+	219	WP_001295676.1	CopG family transcriptional regulator	-
EXA07_RS14210 (2929249)	2929249..2929491	+	243	WP_001087409.1	protein YiiF	-
EXA07_RS14215 (2929821)	2929821..2930750	-	930	WP_216350836.1	formate dehydrogenase accessory protein FdhE	-
EXA07_RS14220 (2930747)	2930747..2931382	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EXA07_RS14225 (2931379)	2931379..2932281	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118614 WP_000897305.1 NZ_CP035819:2927605-2927916 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118614 NZ_CP035819:2927605-2927916 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118614 WP_000356397.1 NZ_CP035819:2927917-2928207 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118614 NZ_CP035819:2927917-2928207 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7