Detailed information of TA system TA118607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 851067..851569
• Replicon: chromosome
• Accession: NZ_CP035819
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EXA07_RS04265
• Protein ID: WP_000767835.1
• Coordinates: 851067..851321 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: EXA07_RS04270
• Protein ID: WP_001259255.1
• Coordinates: 851318..851569 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS04235 (846082)	846082..846309	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
EXA07_RS04240 (846323)	846323..847381	-	1059	WP_000492320.1	thiosulfate utilization transporter TsuA/YeeE	-
EXA07_RS04245 (847560)	847560..848918	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
EXA07_RS04250 (848908)	848908..848970	-	63	WP_010723108.1	membrane protein YoeI	-
EXA07_RS04255 (849185)	849185..850114	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
EXA07_RS04260 (850160)	850160..850984	-	825	WP_000754737.1	SDR family oxidoreductase	-
EXA07_RS04265 (851067)	851067..851321	-	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
EXA07_RS04270 (851318)	851318..851569	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
EXA07_RS04275 (851853)	851853..851903	+	51	WP_001364200.1	his operon leader peptide	-
EXA07_RS04280 (852049)	852049..852948	+	900	WP_000131774.1	ATP phosphoribosyltransferase	-
EXA07_RS04285 (852954)	852954..854258	+	1305	WP_001356284.1	histidinol dehydrogenase	-
EXA07_RS04290 (854255)	854255..855325	+	1071	WP_000108941.1	histidinol-phosphate transaminase	-
EXA07_RS04295 (855325)	855325..856392	+	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T118607 WP_000767835.1 NZ_CP035819:c851321-851067 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T118607 NZ_CP035819:c851321-851067 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT118607 WP_001259255.1 NZ_CP035819:c851569-851318 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT118607 NZ_CP035819:c851569-851318 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0