Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4164414..4164955 Replicon chromosome
Accession NZ_CP035817
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EXA08_RS20520 Protein ID WP_000615976.1
Coordinates 4164677..4164955 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EXA08_RS20515 Protein ID WP_000729704.1
Coordinates 4164414..4164674 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS20490 (4160197) 4160197..4160982 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
EXA08_RS20495 (4160954) 4160954..4162666 + 1713 Protein_4015 flagellar biosynthesis protein FlhA -
EXA08_RS20500 (4162771) 4162771..4163049 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EXA08_RS20505 (4163042) 4163042..4163398 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
EXA08_RS20510 (4163455) 4163455..4164228 - 774 WP_000543895.1 C40 family peptidase -
EXA08_RS20515 (4164414) 4164414..4164674 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EXA08_RS20520 (4164677) 4164677..4164955 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EXA08_RS20525 (4165111) 4165111..4165851 + 741 WP_216264499.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EXA08_RS20530 (4165822) 4165822..4166589 - 768 WP_216264501.1 class II glutamine amidotransferase -
EXA08_RS20535 (4166795) 4166795..4167373 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T118589 WP_000615976.1 NZ_CP035817:4164677-4164955 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118589 NZ_CP035817:4164677-4164955 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT118589 WP_000729704.1 NZ_CP035817:4164414-4164674 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118589 NZ_CP035817:4164414-4164674 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References