Detailed information of TA system TA118574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2202362..2202864
• Replicon: chromosome
• Accession: NZ_CP035817
• Organism: Escherichia coli strain E7476

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EXA08_RS10690
• Protein ID: WP_000767835.1
• Coordinates: 2202610..2202864 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: EXA08_RS10685
• Protein ID: WP_001259255.1
• Coordinates: 2202362..2202613 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA08_RS10660 (2197539)	2197539..2198606	-	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
EXA08_RS10665 (2198606)	2198606..2199676	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
EXA08_RS10670 (2199673)	2199673..2200977	-	1305	WP_001356284.1	histidinol dehydrogenase	-
EXA08_RS10675 (2200983)	2200983..2201882	-	900	WP_000131774.1	ATP phosphoribosyltransferase	-
EXA08_RS10680 (2202028)	2202028..2202078	-	51	WP_001364200.1	his operon leader peptide	-
EXA08_RS10685 (2202362)	2202362..2202613	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
EXA08_RS10690 (2202610)	2202610..2202864	+	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
EXA08_RS10695 (2202947)	2202947..2203771	+	825	WP_000754737.1	SDR family oxidoreductase	-
EXA08_RS10700 (2203817)	2203817..2204746	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
EXA08_RS10705 (2204961)	2204961..2205023	+	63	WP_010723108.1	membrane protein YoeI	-
EXA08_RS10710 (2205013)	2205013..2206371	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
EXA08_RS10715 (2206550)	2206550..2207608	+	1059	WP_000492320.1	thiosulfate utilization transporter TsuA/YeeE	-
EXA08_RS10720 (2207622)	2207622..2207849	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T118574 WP_000767835.1 NZ_CP035817:2202610-2202864 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T118574 NZ_CP035817:2202610-2202864 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT118574 WP_001259255.1 NZ_CP035817:2202362-2202613 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT118574 NZ_CP035817:2202362-2202613 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0