Detailed information of TA system TA118568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 38725..39327
• Replicon: chromosome
• Accession: NZ_CP035817
• Organism: Escherichia coli strain E7476

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EXA08_RS00200
• Protein ID: WP_000897305.1
• Coordinates: 39016..39327 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXA08_RS00195
• Protein ID: WP_000356397.1
• Coordinates: 38725..39015 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA08_RS00170 (34650)	34650..35552	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EXA08_RS00175 (35549)	35549..36184	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EXA08_RS00180 (36181)	36181..37110	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EXA08_RS00185 (37440)	37440..37682	-	243	WP_001087409.1	protein YiiF	-
EXA08_RS00190 (37902)	37902..38120	-	219	WP_001315930.1	CopG family transcriptional regulator	-
EXA08_RS00195 (38725)	38725..39015	-	291	WP_000356397.1	NadS family protein	Antitoxin
EXA08_RS00200 (39016)	39016..39327	-	312	WP_000897305.1	hypothetical protein	Toxin
EXA08_RS00205 (39556)	39556..40464	+	909	WP_001162704.1	alpha/beta hydrolase	-
EXA08_RS00210 (40528)	40528..41469	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EXA08_RS00215 (41514)	41514..41951	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EXA08_RS00220 (41948)	41948..42820	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EXA08_RS00225 (42814)	42814..43413	-	600	WP_001295269.1	glucose-1-phosphatase	-
EXA08_RS00230 (43512)	43512..44297	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118568 WP_000897305.1 NZ_CP035817:c39327-39016 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118568 NZ_CP035817:c39327-39016 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118568 WP_000356397.1 NZ_CP035817:c39015-38725 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118568 NZ_CP035817:c39015-38725 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7