Detailed information of TA system TA118547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 947695..948297
• Replicon: chromosome
• Accession: NZ_CP035812
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EXA09_RS04340
• Protein ID: WP_000897305.1
• Coordinates: 947986..948297 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXA09_RS04335
• Protein ID: WP_000356397.1
• Coordinates: 947695..947985 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS04310 (943621)	943621..944523	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EXA09_RS04315 (944520)	944520..945155	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EXA09_RS04320 (945152)	945152..946081	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EXA09_RS04325 (946411)	946411..946653	-	243	WP_001086388.1	protein YiiF	-
EXA09_RS04330 (946872)	946872..947090	-	219	WP_001297075.1	CopG family transcriptional regulator	-
EXA09_RS04335 (947695)	947695..947985	-	291	WP_000356397.1	NadS family protein	Antitoxin
EXA09_RS04340 (947986)	947986..948297	-	312	WP_000897305.1	hypothetical protein	Toxin
EXA09_RS04345 (948526)	948526..949434	+	909	WP_001162704.1	alpha/beta hydrolase	-
EXA09_RS04350 (949498)	949498..950439	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EXA09_RS04355 (950484)	950484..950921	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EXA09_RS04360 (950918)	950918..951790	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EXA09_RS04365 (951784)	951784..952383	-	600	WP_001295269.1	glucose-1-phosphatase	-
EXA09_RS04370 (952482)	952482..953267	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118547 WP_000897305.1 NZ_CP035812:c948297-947986 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118547 NZ_CP035812:c948297-947986 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118547 WP_000356397.1 NZ_CP035812:c947985-947695 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118547 NZ_CP035812:c947985-947695 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7