Detailed information of TA system TA118484

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2356287..2356658
• Replicon: chromosome
• Accession: NZ_CP035772
• Organism: Escherichia coli O145 strain RM8843-C1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: EW648_RS11705
• Protein ID: WP_021500490.1
• Coordinates: 2356464..2356658 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2356287..2356465 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW648_RS11680	2352241..2353614	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
EW648_RS11685	2353743..2354678	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EW648_RS11690	2354731..2355966	-	1236	WP_000040838.1	site-specific integrase	-
EW648_RS11695	2355968..2356183	-	216	WP_000079604.1	excisionase XisR	-
EW648_RS11700	2356283..2356471	-	189	WP_001298826.1	DUF1187 family protein	-
-	2356287..2356465	+	179	NuclAT_0	-	Antitoxin
-	2356287..2356465	+	179	NuclAT_0	-	Antitoxin
-	2356287..2356465	+	179	NuclAT_0	-	Antitoxin
-	2356287..2356465	+	179	NuclAT_0	-	Antitoxin
EW648_RS11705	2356464..2356658	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EW648_RS11710	2356722..2357774	-	1053	WP_001004423.1	recombinase RecT	-
EW648_RS11715	2357786..2359837	-	2052	Protein_2294	PD-(D/E)XK nuclease-like domain-containing protein	-
EW648_RS11720	2359891..2361104	+	1214	WP_167703748.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	2354731..2408075	53344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T118484 WP_021500490.1 NZ_CP035772:c2356658-2356464 [Escherichia coli O145]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T118484 NZ_CP035772:c2356658-2356464 [Escherichia coli O145]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT118484 NZ_CP035772:2356287-2356465 [Escherichia coli O145]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin