Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1612622..1613124 Replicon chromosome
Accession NZ_CP035772
Organism Escherichia coli O145 strain RM8843-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EW648_RS07650 Protein ID WP_000767829.1
Coordinates 1612870..1613124 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EW648_RS07645 Protein ID WP_001259255.1
Coordinates 1612622..1612873 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW648_RS07620 1607799..1608866 - 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EW648_RS07625 1608866..1609936 - 1071 WP_001099213.1 histidinol-phosphate transaminase -
EW648_RS07630 1609933..1611237 - 1305 WP_001400693.1 histidinol dehydrogenase -
EW648_RS07635 1611243..1612142 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EW648_RS07640 1612288..1612338 - 51 WP_001364200.1 his operon leader peptide -
EW648_RS07645 1612622..1612873 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EW648_RS07650 1612870..1613124 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EW648_RS07655 1613207..1614031 + 825 WP_000754755.1 SDR family oxidoreductase -
EW648_RS07660 1614077..1615006 + 930 WP_000803351.1 LysR family transcriptional regulator -
EW648_RS07665 1615221..1615283 + 63 WP_010723108.1 membrane protein YoeI -
EW648_RS07670 1615273..1616631 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EW648_RS07675 1616810..1617868 + 1059 WP_000492332.1 transport protein YeeE -
EW648_RS07680 1617882..1618109 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T118482 WP_000767829.1 NZ_CP035772:1612870-1613124 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T118482 NZ_CP035772:1612870-1613124 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118482 WP_001259255.1 NZ_CP035772:1612622-1612873 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118482 NZ_CP035772:1612622-1612873 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References