Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3879582..3880309 Replicon chromosome
Accession NZ_CP035770
Organism Escherichia coli O145 strain RM8988-C1

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag EW646_RS19250 Protein ID WP_000550189.1
Coordinates 3879995..3880309 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EW646_RS19245 Protein ID WP_000560266.1
Coordinates 3879582..3879998 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW646_RS19235 3875671..3877215 + 1545 WP_000258466.1 hypothetical protein -
EW646_RS19240 3877519..3879537 + 2019 WP_000121443.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EW646_RS19245 3879582..3879998 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
EW646_RS19250 3879995..3880309 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
EW646_RS19255 3880594..3881730 - 1137 WP_000018698.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EW646_RS19260 3881815..3882318 + 504 WP_001295542.1 M48 family metallopeptidase -
EW646_RS19265 3882395..3883087 + 693 WP_000942541.1 vancomycin high temperature exclusion protein -
EW646_RS19270 3883166..3884152 + 987 WP_024221603.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T118472 WP_000550189.1 NZ_CP035770:c3880309-3879995 [Escherichia coli O145]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T118472 NZ_CP035770:c3880309-3879995 [Escherichia coli O145]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT118472 WP_000560266.1 NZ_CP035770:c3879998-3879582 [Escherichia coli O145]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT118472 NZ_CP035770:c3879998-3879582 [Escherichia coli O145]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATTTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References