Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2630164..2630666 Replicon chromosome
Accession NZ_CP035770
Organism Escherichia coli O145 strain RM8988-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EW646_RS13325 Protein ID WP_000767829.1
Coordinates 2630164..2630418 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EW646_RS13330 Protein ID WP_001259255.1
Coordinates 2630415..2630666 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW646_RS13295 2625179..2625406 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EW646_RS13300 2625420..2626478 - 1059 WP_000492332.1 transport protein YeeE -
EW646_RS13305 2626657..2628015 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EW646_RS13310 2628005..2628067 - 63 WP_010723108.1 membrane protein YoeI -
EW646_RS13315 2628282..2629211 - 930 WP_000803351.1 LysR family transcriptional regulator -
EW646_RS13320 2629257..2630081 - 825 WP_000754755.1 SDR family oxidoreductase -
EW646_RS13325 2630164..2630418 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EW646_RS13330 2630415..2630666 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EW646_RS13335 2630950..2631000 + 51 WP_001364200.1 his operon leader peptide -
EW646_RS13340 2631146..2632045 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EW646_RS13345 2632051..2633355 + 1305 WP_001400693.1 histidinol dehydrogenase -
EW646_RS13350 2633352..2634422 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
EW646_RS13355 2634422..2635489 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T118467 WP_000767829.1 NZ_CP035770:c2630418-2630164 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T118467 NZ_CP035770:c2630418-2630164 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118467 WP_001259255.1 NZ_CP035770:c2630666-2630415 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118467 NZ_CP035770:c2630666-2630415 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References