Detailed information of TA system TA118443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3425479..3426206
• Replicon: chromosome
• Accession: NZ_CP035768
• Organism: Escherichia coli O145 strain RM11626-C1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EW649_RS17040
• Protein ID: WP_000547563.1
• Coordinates: 3425895..3426206 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EW649_RS17035
• Protein ID: WP_000126294.1
• Coordinates: 3425479..3425898 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW649_RS17015	3420528..3421055	-	528	WP_001078777.1	electron transport protein HydN	-
EW649_RS17020	3421204..3422217	-	1014	WP_024221754.1	DNA-binding transcriptional regulator AscG	-
EW649_RS17025	3422475..3423932	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EW649_RS17030	3423941..3425365	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
EW649_RS17035	3425479..3425898	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EW649_RS17040	3425895..3426206	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EW649_RS17045	3426368..3427123	-	756	WP_001400787.1	hypothetical protein	-
EW649_RS17050	3427166..3427633	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
EW649_RS17055	3427626..3428036	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
EW649_RS17060	3428033..3428800	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
EW649_RS17065	3428800..3429342	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EW649_RS17070	3429352..3431061	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T118443 WP_000547563.1 NZ_CP035768:c3426206-3425895 [Escherichia coli O145]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T118443 NZ_CP035768:c3426206-3425895 [Escherichia coli O145]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT118443 WP_000126294.1 NZ_CP035768:c3425898-3425479 [Escherichia coli O145]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT118443 NZ_CP035768:c3425898-3425479 [Escherichia coli O145]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22