Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1991264..1991766 Replicon chromosome
Accession NZ_CP035768
Organism Escherichia coli O145 strain RM11626-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EW649_RS09940 Protein ID WP_000767829.1
Coordinates 1991264..1991518 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EW649_RS09945 Protein ID WP_001259255.1
Coordinates 1991515..1991766 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW649_RS09910 1986279..1986506 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EW649_RS09915 1986520..1987578 - 1059 WP_000492332.1 transport protein YeeE -
EW649_RS09920 1987757..1989115 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EW649_RS09925 1989105..1989167 - 63 WP_010723108.1 membrane protein YoeI -
EW649_RS09930 1989382..1990311 - 930 WP_000803351.1 LysR family transcriptional regulator -
EW649_RS09935 1990357..1991181 - 825 WP_000754755.1 SDR family oxidoreductase -
EW649_RS09940 1991264..1991518 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EW649_RS09945 1991515..1991766 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EW649_RS09950 1992050..1992100 + 51 WP_001364200.1 his operon leader peptide -
EW649_RS09955 1992246..1993145 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EW649_RS09960 1993151..1994455 + 1305 WP_001400693.1 histidinol dehydrogenase -
EW649_RS09965 1994452..1995522 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
EW649_RS09970 1995522..1996589 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T118441 WP_000767829.1 NZ_CP035768:c1991518-1991264 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T118441 NZ_CP035768:c1991518-1991264 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118441 WP_001259255.1 NZ_CP035768:c1991766-1991515 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118441 NZ_CP035768:c1991766-1991515 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References