Detailed information of TA system TA118419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3425382..3426109
• Replicon: chromosome
• Accession: NZ_CP035767
• Organism: Escherichia coli O145 strain RM12522-C8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EW647_RS17490
• Protein ID: WP_000547563.1
• Coordinates: 3425798..3426109 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EW647_RS17485
• Protein ID: WP_000126294.1
• Coordinates: 3425382..3425801 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW647_RS17465	3420431..3420958	-	528	WP_001078777.1	electron transport protein HydN	-
EW647_RS17470	3421107..3422120	-	1014	WP_024221754.1	DNA-binding transcriptional regulator AscG	-
EW647_RS17475	3422378..3423835	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EW647_RS17480	3423844..3425268	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
EW647_RS17485	3425382..3425801	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EW647_RS17490	3425798..3426109	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EW647_RS17495	3426271..3427026	-	756	WP_001400787.1	hypothetical protein	-
EW647_RS17500	3427069..3427536	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
EW647_RS17505	3427529..3427939	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
EW647_RS17510	3427936..3428703	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
EW647_RS17515	3428703..3429245	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EW647_RS17520	3429255..3430964	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T118419 WP_000547563.1 NZ_CP035767:c3426109-3425798 [Escherichia coli O145]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T118419 NZ_CP035767:c3426109-3425798 [Escherichia coli O145]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT118419 WP_000126294.1 NZ_CP035767:c3425801-3425382 [Escherichia coli O145]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT118419 NZ_CP035767:c3425801-3425382 [Escherichia coli O145]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22